FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5a3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5a3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences874608
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA15520.17745092658653933No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC14900.17036203647805645No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC12830.1466942904707023No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA12330.14097744360902253No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT10880.12439858771015129No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT10020.11456561110806214No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT9810.11216453542615663No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC9440.1079340687485136No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT9430.10781973181128003No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG9430.10781973181128003No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT8860.10130252638896511No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT11100.043.118534
TTCCGAT11350.041.5251122
CTTCCGA11450.041.162451
TCGCGCG5400.037.5930829
CGATCTG5900.036.8733185
GTCGCGC5700.035.61458
GGTCGCG6100.033.279127
TATAGGT6550.032.1073
AGGTCGC7150.030.4269356
TACGAGT7750.027.135594
TCCCCCC5900.025.805428
CTTGCGA4001.8189894E-1225.3811343
TGCGACC3801.2732926E-1124.8086285
ACCATAC7600.024.8057921
GTATATA4800.024.1697461
CCTATAA12700.023.9794311
CCCCCCG6350.023.976699
TCCTACA6050.023.9699952
GTATTAT3052.0310836E-823.773521
CCTACAC5850.023.55273
Produced by FastQC (version 0.11.2)