FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563920
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC13490.23921832884097036No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA12810.22715988083416086No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA10420.1847779826925805No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC10150.17999006951340615No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT9620.17059157327280466No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG8380.14860263867215207No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT7890.13991346290253937No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT7800.13831749184281458No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT7750.1374308412540786No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT7630.13530287984111222No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC7450.13211093772166263No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT7080.12554972336501632No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG6740.11952049936161156No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA6270.11118598382749326No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG6240.11065399347425166No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA6190.10976734288551569No Hit
GTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGA5830.10338345864661654No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT5660.10036884664491416No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT6200.043.2764784
CTTCCGA7100.039.8299331
TCCCCCC4050.037.5915838
CGATCTG3900.035.328965
TTCCGAT7850.035.1008222
TCGCGCG4400.034.6013459
AGGTCGC4750.033.5869836
GTCGCGC4650.032.7410558
TATAGGT4900.032.5588073
GGTCGCG5000.031.8991417
CTAGGGG1153.1559326E-431.5292783
CCCCCCG4850.031.3909079
CCATACT5450.030.6009332
ATACTCC5300.028.7332864
GTATTAT1555.4905948E-528.068741
ACCTATA5250.027.6232031
CCTATAA10250.027.5895161
TACTCCC6400.027.1940025
CTCCCCC5700.026.709817
TTAGACA2456.413029E-826.6390254
Produced by FastQC (version 0.11.2)