FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5a1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5a1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGGG10.7194244604316548No Hit
GGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACA10.7194244604316548No Hit
ATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCT10.7194244604316548No Hit
GGTAGGACGTGTCGGCTGCTTTGTTGACCCGTCACACGGAAACGAGAGCT10.7194244604316548No Hit
CACAAGAGGTAATCAATAAAATGCAGGCTCCGAATGTTTAATAAAAACAA10.7194244604316548No Hit
GCAAAACATTGCGCAAATGGCAGCCGAGGAAGGGATTGATTTTCAATGGC10.7194244604316548No Hit
CCTTCTGGATGTTGTAATCTGCAAGTGTGCGACCATCCTCAAGCTGTTTT10.7194244604316548No Hit
TATATATCATTTAAACTAATTAAAAGCCAGAATCGTTCTTCATAACCAAA10.7194244604316548No Hit
AAGGGTGTTGGTCGATTAAGACAGCACGACGGTGGTCATGGAAGTCGAAA10.7194244604316548No Hit
ATACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAA10.7194244604316548No Hit
GAATTGTAGTCTGGAGAAGCGTCCTCAGCGACGGACCGGGCCTAAGTTCC10.7194244604316548No Hit
GGGGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTTAGTCGATCCTA10.7194244604316548No Hit
TCCTCGTGCTCCTCAAAGAACATTTTCCACAAAAATGATCTGAATGGAGC10.7194244604316548No Hit
AGACAGAATCCCGGCAAGAAGCGACGCAAGCACCCGCCCCCCGAATTCCG10.7194244604316548No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT10.7194244604316548No Hit
TCTCGAACTCGTACAGCTCGCCCTCGGCGGTGTCCAGCGGCTCGATCGCA10.7194244604316548No Hit
AGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGACTAGGA10.7194244604316548No Hit
GCCCTGTACAGAGCTGCTAGTAAGACGTTTTACAGCTATTTCCTTTCCGT10.7194244604316548No Hit
CAAGTGGGGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCAT10.7194244604316548No Hit
GGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA10.7194244604316548No Hit
ATAAAAAGCAAACTAAAACACACATTACAAATACCTTAATCTTAAAATCG10.7194244604316548No Hit
GATCTGCGGCGGCGGAAGCGAGGTTTTCGTTGGGGAGAGAGACAACGACT10.7194244604316548No Hit
GCTTAAGGGCAGTTTCTTAGTTAAAAGTAACCAAGTTTACATGTTTGAGC10.7194244604316548No Hit
AGCGGTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGG10.7194244604316548No Hit
CAGATCATCAGATCACAAAATACTATTAACTAATTAAAACAGATATCACA10.7194244604316548No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC10.7194244604316548No Hit
GTTATAGCATAATGCAAGTGTTCCAATCGCCATGATCTGAGGGATGGCAC10.7194244604316548No Hit
TTACCCCACTCAGGCATAGTACACCATCTTTCGGGTCCCGACAGGCATGC10.7194244604316548No Hit
AATAATCGCCTACCCAAACGACAATACCACCAATGCACACCAAACGCGCA10.7194244604316548No Hit
TTTGTAGTCAGAAGCCGCATTGGCGTGGACAAAACCGCAGCAATCGGCCC10.7194244604316548No Hit
AAATAGAACTCGTCGTTGGGCTGCATGCCGCTGAAGGACGCCATGCGGTT10.7194244604316548No Hit
GAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACGTCT10.7194244604316548No Hit
CATGTGAACGGCACTTGCACATGGGTTAGTCGATCATAAGAGTCGGGGGA10.7194244604316548No Hit
CAATAAATATGTACAACGAATCCGCAAAAATCTAAAGACTTCAAACCAAA10.7194244604316548No Hit
TCATAACACTGCTCTGTGGCGACCATCTTGGCCATAGCGCACAATACGGT10.7194244604316548No Hit
CCTTGGGATGGGTCGGCCGGTCCGCCTTTGGTGTGCATTGGTCGGCTTGT10.7194244604316548No Hit
CTTCTTCCACAGGAGGAGGAACAACCACCGGTGCCGCAGCGACGGCTGAA10.7194244604316548No Hit
CAGCAGAACTGGCGATGCGGGATGAACCGGAAGCCGGGTTACGGTGCCCA10.7194244604316548No Hit
CGATTGGGGCTGCATTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTG10.7194244604316548No Hit
TTCCACCAGAGTTTCCTCTGGCTTTACCCCGCTCAGGCATAGTTCACCAT10.7194244604316548No Hit
TTGCTGTGGTGCAACGTATGTCCTAGTTGCTGGAACACTAGACGAATCAA10.7194244604316548No Hit
CGTCGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTG10.7194244604316548No Hit
TAAAAACACAATTAAATTTAACAAAATACAAAAGAATCTCAAATTTAACA10.7194244604316548No Hit
AAATAACAATAACTGTATCTTTCTAGTCAGTTAATAAGAATCAAAAAAAT10.7194244604316548No Hit
GTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGA10.7194244604316548No Hit
GCTCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATGGT10.7194244604316548No Hit
CTCATATACGAATACAAAAAACAAATAATTCATCCTCGTTAGTAGTATTC10.7194244604316548No Hit
CGGATGATGAGTTCGATGGAGGAACTTCGCGGAGGAAACTATAATCTTGA10.7194244604316548No Hit
TATAAACCGCAAAAGGTTAAATTACTTAACATTGACATTCAGATAACTGG10.7194244604316548No Hit
CAATTTCACCTCTGACTATGAAATAAGAATGCCCCCCACTGTCCCTGTTA10.7194244604316548No Hit
ATTTGGAAGAACCCTTCATTGTACCCTTACGTTCATTACATGAAGCAACG10.7194244604316548No Hit
ATTTGCACGTCAGTATCGCTGCGGGCTTCCACCAGAGTTTCCTCTGGCTT10.7194244604316548No Hit
CATCTTTACCTCGCGGTACTAGTTCGCTATAGGTCTCTCGCCCGTATTTA10.7194244604316548No Hit
GGGATTTAGATTGTACTCATTCCAATTACCAGACTCGAAAGAGCCCGGTA10.7194244604316548No Hit
GAAATACCCCTGGCCATATTACGAGAAATAGGGCTTGACCAAAAAAACTC10.7194244604316548No Hit
ATAATGACCACATCAGCTGATGAAGGACAGTTCTTAAACATGCTTATCAA10.7194244604316548No Hit
GATCCCTGGTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCG10.7194244604316548No Hit
GCGAGAACTTGTGTGTACCCGGCAAGCCGGATCACTTAACAAAGACGCGT10.7194244604316548No Hit
GCACAACAATATAACGTACAAACGGAAACAAAATGAATAATTAATACAAA10.7194244604316548No Hit
GTGCTGAATCTGATCTTGAGTCAATCCATAAGCGTGGTAGTCTTAGTGAA10.7194244604316548No Hit
TTTGAGAATAGGTCGAGGGCGTTGCGCCCCCAATGCCTCTAATCATTGGC10.7194244604316548No Hit
CACCTATCACACTTCACCGGCACCAGTATCCGACGACAAGACGGACACGA10.7194244604316548No Hit
CGATAGAACTCGCGTCCGAGCTCCAGCTATCCTGAGGGAAACTTCGGAGG10.7194244604316548No Hit
GGTGAAGGAGGACCAGCATCTGGAAGCCTTCCTTCTTCTGGACAATCCTC10.7194244604316548No Hit
AAACTGACGTGCAAATCGTTCGTCTGAATTGGGTATAGGGGCGAAAGACT10.7194244604316548No Hit
TTGTTAGCTCACGCAAAGAATCTCTTCAGCACTTCAAGCAGCCATGGGCA10.7194244604316548No Hit
TTGACATTCAGAGCACTGGGCAGAAATCACATTGCGTTAGCATCCGCAGG10.7194244604316548No Hit
ATCAAATGGATCCTCGTTAAGGGATTTAGATTGTACTCATTCCAATTACC10.7194244604316548No Hit
ACAAATAGGTTCTCAAATAATTATAAGATGGTATGCTAGGTCTGTCTCTT10.7194244604316548No Hit
CTACTACGTTGATTTCAAAAATAACCTCACACGTTACGAGAAAGAATCCG10.7194244604316548No Hit
ATAGAACTCGCGTCCGAGCTCCAGCTATCCTGAGGGAAACTTCGGAGGGA10.7194244604316548No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.7194244604316548No Hit
GAGCCTAAGATGCCACCTCTACAGTACCCAAAGGTGTAGCATTTACCTGC10.7194244604316548No Hit
ACACTCCTTAGCGGAATTCGACTTCCATCACCACCTGTCTCTAATACACA10.7194244604316548No Hit
AAATACCATTTATGGGTATTTCAACCGAGATACCTGACTGCGGTATGAAT10.7194244604316548No Hit
CGCACGTCGCGTGGTGTCCGGTGCGCACCCGGCGGGCCGTGAATATCCGG10.7194244604316548No Hit
CAAATGATCATATCCAAAGCTAAAGTATCCACAGCCTAAGCTTCATAACA10.7194244604316548No Hit
GGACAGTATCGCCCACCCAACCGAAAAGCAGAACAATTCACAAAAAACCA10.7194244604316548No Hit
AATTAGCATGGGATAACATCATAGGATTTCGATCCTATTTTTTTGGCATT10.7194244604316548No Hit
AACAGGTACAGTGATTGGAGCACCAGTGTTGAGAACCTTCCTTCCACGGA10.7194244604316548No Hit
GCGGGGGATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTGG10.7194244604316548No Hit
GCATACAACCCTGGTTTTTCTCTATTGGCCGTTTGCATTTTGGACAGGGC10.7194244604316548No Hit
ATTCTGATGTCTCCTTAGAAGTAACCACCGGTTTTTCTAACAGATAAAAC10.7194244604316548No Hit
GTGATGAAGGATGTTCCTGGATGGAAAGTCGGTGAAAACGTGTACAATTC10.7194244604316548No Hit
ATCTAATAAAAAACAATAAATTAGATAAAAAAACAACTTTAATATAAAAC10.7194244604316548No Hit
ATCTTCCCGAGGCCCCCGCCGACGTCTCCGGACTCCCTAACGTTGCCGTC10.7194244604316548No Hit
TCTGAGAAGGGTTCGAGAGTGAGCATGCCTGTCGGGACCCGAAAGATGGT10.7194244604316548No Hit
GCGCCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGCCCCTGTCGCACCACG10.7194244604316548No Hit
ATCATTGCGACCAAAAAAATTCACTACTTCAGCAATCACCTAACCACGTA10.7194244604316548No Hit
CTTCACCAGAGTTCTAACAAACTCCTTAGATTAATACTAGATCAATCAAC10.7194244604316548No Hit
TATCACACAACACTAAAAAATAATCCAACAACATCGAAAAAATAATACCT10.7194244604316548No Hit
TTATGAAGTAGCGCCAGACAAAGCAACACAATAACAGCAAGCCCTCGACG10.7194244604316548No Hit
GCAGAAAGTATAAAGAGAAAACATAATAGTAGTTACAAAATATCTTATAC10.7194244604316548No Hit
AACTTGGAGAACAGCTAGAAAGACAAAAAAAAAGAGACAATGATCAACAA10.7194244604316548No Hit
AAAAAAAAAAAAAAAAAAAAAAACAAAACAAAAAAAAAAAAAAAAAAAAA10.7194244604316548No Hit
GAGTCGTCTTGTTATTTTCCCAAATCTGATGGCAGAGGAGAATCCACCTC10.7194244604316548No Hit
GCCGCCAAGGGCTGAAACGCCCGGCTATCGGCCTGATTTTGCGGCCGCCG10.7194244604316548No Hit
ACCTTGAACTACTAACAAGTGTAAAACCGATAAAATTAATCAAATCACAA10.7194244604316548No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTA10.7194244604316548No Hit
GGCGCGTTCCCTTCGACGCCAACCGTTTCCGCGGCTACTCGACCATCAAC10.7194244604316548No Hit
CCACCTTCACTACTAACCCTTTTGTTTCCTCCTTCAAGTCCAGGTTTTGA10.7194244604316548No Hit
GTCATTAGTATCTTGATAGGGAATTAATTGAATCTGTATATTGCTATGGG10.7194244604316548No Hit
GTCCCCAAGCAACCCAAGAAGAAGCAGAGGTTCCAGGGTGGCGATAGGCG10.7194244604316548No Hit
CCCTTTGACTCTAAAAACACATCACTACTGATCTATTTTATGTCGAAAAA10.7194244604316548No Hit
GATTTAGTTTGTATCTTTTTTGGAATCGGTCTGAAGTTGGACAAAAGAAT10.7194244604316548No Hit
TTGGTAGATTTTGCACCTGATCCGATGGGGCGTAAGTCTTTCCCTTGACT10.7194244604316548No Hit
GTTAGTAGAGAGTTAGGCTACATGCGGTAAGGTACTTTTTCATGCATCAA10.7194244604316548No Hit
CTACCAATGTGATGGATGAAAATTCCTTCTCACAGCCAGAGAAGGTTGTA10.7194244604316548No Hit
AGGTAAAGATGAAAAGGACTTTGAAAAGAGAGTCAAAGAGTGCTTGAAAT10.7194244604316548No Hit
CTATTGAACTAAACCCCCGCTGATGATAGGTGTTCCCTTGCGTTTAAAGA10.7194244604316548No Hit
GTCATAAATAACCTGCAAATGCTAGCCACAGAAGATTAGCACCCAGTAGA10.7194244604316548No Hit
AACGAGTTCCACACACCCAACCTCAAAAACATAACATAATAAGGAATACA10.7194244604316548No Hit
CATCAACGTCTCCCACCTATCCTACACAAACCGTACCAATCACCAATACC10.7194244604316548No Hit
GCGTAGTACTTCAAGAAAAAGGCGAGCAGAACACCGATGAACTACATGCT10.7194244604316548No Hit
CTACATAACCCATTACGACAACCATTCCAAAATTCCTCCAAACAAAATCT10.7194244604316548No Hit
TCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGTCTC10.7194244604316548No Hit
GATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGAAG10.7194244604316548No Hit
GCAAATATTCAGTCACAATCATCATTGGGTTACTTTGGGTTACAGCACCA10.7194244604316548No Hit
CCGCAGGAGGTTTCACGAGGGAAGATGGGAATGAGGCTGTGTCAAAGGGA10.7194244604316548No Hit
GGGTTAGTCGATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCGCTTAAG10.7194244604316548No Hit
CTCTAATTTCTTCAAAGTAACAGCGACGGAGGCACGACCCGGCCAATTAA10.7194244604316548No Hit
GTAGGATAGTGGCATACCAAGGGGGTAACTGGTGAAGGATAGTTAGGTGT10.7194244604316548No Hit
CCCGCGTCCTGCGGCCGGGTCCAGTTCGCCGAGCAGCTCGGCGGCCTCGT10.7194244604316548No Hit
ATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTGA10.7194244604316548No Hit
GTTAATTGAACGTTCGGCGTATGAGTGGTGATCGGATAGCCAGTGTTCGT10.7194244604316548No Hit
GACTAGGACGGTATCTGATAGTCTTCGAGCCCCCAACTTTATTTCTTGAT10.7194244604316548No Hit
CCTTTTGAATGTTGTAGTCAGCAAGAGTTCTTCCGTCTTCAAGCTGCTTA10.7194244604316548No Hit
CCTGGAGGAAGAACATTGTCCACGAATATGAAGATAGCCTTTTCTGCACA10.7194244604316548No Hit
AGCTTCTCTAGAGGATTACGAGCAAGGTTTGAAAGCTTGCGAGGAAGCAG10.7194244604316548No Hit
TATATATTTAGGATAGTTAGATCTTCTTGTTGAATTGAACCATTTACATT10.7194244604316548No Hit
AAAAATAAACTCAATAACTGTCAATAAAAAGTACTATAAAACTATCAGTC10.7194244604316548No Hit
AATCTGCATCTTGCACTGGCTTTGTCCTGTCTTGTGGCAGGAAGTGCTGG10.7194244604316548No Hit
TACAAGTACCGTGAGGGAAAGGTGAAAAGAACCCTATGACGGGAGTGCAA10.7194244604316548No Hit
AGTGGGAGCTTCGGCGCAAGTGAAATACCACTACTTTTAACGTTAGTTTA10.7194244604316548No Hit
AACTAAGACTCTCTTAAGGTAGACAAATGCCTCGTCAACTAATTAGTGAC10.7194244604316548No Hit
AACAAATACATAAAATCCAACAGTAAAAAAAATAACATTTACTAAATCCA10.7194244604316548No Hit
AAAAAGTACCGAGAGTTAAAGATGAAAAGGAGTTTGAAAAGAGAGTAAAA10.7194244604316548No Hit
GAAATAAAACAACAAATTAACCAAAATACAATAAAACAAAAATTATAAAC10.7194244604316548No Hit
AGTTTCCCTCAGGATAGCTGGAGCTCGGACGCGAGTTCTATCGGGTAAAG10.7194244604316548No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers