FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5a12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5a12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66.382978723404255No Hit
ATCCATTCATGCGCGTCACTAATTAGATGACGAGGCATTAGGCTACCTTA11.0638297872340425No Hit
ACGGGACTCCCACCCACTACGGTCTGGCACTCACCACCAGTTCGACTACA11.0638297872340425No Hit
TGTCTTAAGGTTCTGGCGCCTGCCAGCCTCGATGTGCGCAGAGTGGGGCG11.0638297872340425No Hit
CGCGTATCCAGCGCCAGCGCCACCAGGTGCACGTGCATGTCGGGCTGCGG11.0638297872340425No Hit
GTGATGACGTCCACGGACGGGCGCTGGGTGGCCATGATCAGGTGGATGCC11.0638297872340425No Hit
GTGTAGCTGTCCGGGCTGCAGTAGTCGTAGTGGGCGAGGGCCGCCAATGA11.0638297872340425No Hit
TTGATATCCTTCATCGTCTTACCACGCATCTCCAGGGGGAAGAGTTGTAT11.0638297872340425No Hit
ATATTCGGAGAAGGCGATGAAGTCGGAGACAGAGTAGATTTGCCGATTAC11.0638297872340425No Hit
GGTGGAGGAATCCCATTCCGACGGGCTCCTGGAGTACCCCGTCTACACCA11.0638297872340425No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC11.0638297872340425No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC11.0638297872340425No Hit
ATTTGCACGTCAGTATCGCTGCGGGTTTCCACCAGAGTTTCCTCTGGCTT11.0638297872340425No Hit
CTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTTCC11.0638297872340425No Hit
GTCCAGCGGTACAGCTCCGCAGTGATCGACGGCGACCTCGCAACTGCCGA11.0638297872340425No Hit
GAATTATTATCGTCTTCATGGCTTGTTTATTAGAAACATAGATCACTCAC11.0638297872340425No Hit
GTTCACCATCTTTCGGGTCCCGACAGGCATGCTCACACTCGAACCCTTCT11.0638297872340425No Hit
GGAAGGGGGCCTCGCCTTCCTCGACAAAGGCGCCATTGGCCATGCGACAA11.0638297872340425No Hit
TATCAAAGTATAAAATATTAGCACGAATTAAAAAACCATCCACACAACTC11.0638297872340425No Hit
GAGTAGCAGGGCCCGAGGCACCCATGAATAACCAGGCCCCAAACCATTTA11.0638297872340425No Hit
AATTACAATGCAAGGAAACTTAAAAACCACAGAGTTAAAGATATACGAAT11.0638297872340425No Hit
CCCTATCATTCGAGATCTGCTTCTTAGCTTAGGCCTTTGACATGTGGACG11.0638297872340425No Hit
GCCATGGCGTGCGCCAGCGAAAGGCCCGAGACGCGCGCGGCATAGGCGTT11.0638297872340425No Hit
GGTTTACTCCGGTTTGGCTATAAACTGTTTGCTTCTCTTGAGTACTCGAG11.0638297872340425No Hit
GATCTATTCAGGGTGACAGTGTTTTGAGCAAAATGACGACCACCATTTCA11.0638297872340425No Hit
CTTATCGAAGGCTACAAACTGTCGACAACCAAAGCGTTCATTCAGTAAAT11.0638297872340425No Hit
TAAAAGAACTGCATCGACATTCCGTAACTTCGTTTCAAAAGATTCCAATT11.0638297872340425No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.0638297872340425No Hit
ACTGTTTTCTTAAAGTTCAAAAAATATGAATGATCCGTCCACATATGAAG11.0638297872340425No Hit
CATACCACACACAATCAACAGGAGTTTTAAGCACAGACCACAACTACAAC11.0638297872340425No Hit
CAACAATACATTAAAATCCAACAGTATAATATCCTGTGCCGATACGAGTG11.0638297872340425No Hit
GATCATGAGGCTTTGCCCTCACGAATGGATGAATGCCATTATTGCAGGAG11.0638297872340425No Hit
GGATCCCGCGCCATCTTTGGTGTCACTCTTGGCATCGCGCTCAGGTTTGA11.0638297872340425No Hit
GAGTTGTTACACACTCCTTAGCGGATTTCGAATTCCATGACCACCGTCCT11.0638297872340425No Hit
ACCATCACATTATGATAACTTAACACAAAGACAATATTATATTGATAAAG11.0638297872340425No Hit
CATCCACCCCGATCCCGAAGAACTGGGCAGCGTGTACCAGGGCGAACTGA11.0638297872340425No Hit
AAATGTCAAAGTGAAGAAAATCAACCAAGCGCGGGTAAACGGCGGGAGTA11.0638297872340425No Hit
GTTGTATAATAGATATTGATAAATCAGATTATGATTTATATTATAAAATA11.0638297872340425No Hit
ACGCGAGTTCTATAGGGTAAAGCCAATGATTAGAGGCAATGGGTGCGCAA11.0638297872340425No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGG11.0638297872340425No Hit
AGATTAAAAATAAAATTGAAACAAGATAAAATCGTATATCGAAACGACTT11.0638297872340425No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.0638297872340425No Hit
GTACCCTTGACTTACGTCGGCAAGAACATAACCATATCCCAAGACCCACC11.0638297872340425No Hit
GCGTTAGGGAGTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTT11.0638297872340425No Hit
TCTTCATTCAAATCGAGATGGCCCGCAAAAAGGTCTTGCTCTTTGAAGAC11.0638297872340425No Hit
CTATTTGCAGAGCTAAATGTCTCAATCACTTCCCCAAATACTGACAATCT11.0638297872340425No Hit
GAAAACCGATGGACCCTGCGGATGCGCACGGAATGGCACTTCGGCCCCGT11.0638297872340425No Hit
GGAATATTCACCTGTTTCCCATCAGCTACGCATCTCTGCCTCGCCTTAGG11.0638297872340425No Hit
GTTCGTCCACGAGCCCGGCTTCCAGGAAGGCGCCGGACAGCGTCGGGCCA11.0638297872340425No Hit
CAGCCAGACTGGTGGCTTCGACGTAGCGGCCATTCTCGACCACGCCAGGA11.0638297872340425No Hit
GGTGTCCGGTGCGCCCCCGGCGGCCCTTGAACATGCGGAGGACCGAGTGC11.0638297872340425No Hit
TAACAGAAGAAGGCCAAACTGCTGTTCAGACGTTTCGCCAAGCTTAAGCC11.0638297872340425No Hit
CCAAATAGAAATAAGCCTTAATTGATTCAAAAACATCATTAAAAAAGCAA11.0638297872340425No Hit
CGTCACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATAGT11.0638297872340425No Hit
ATACATTCGGCTGCGGCTGTGCCCACCATCTTGGTATGCAACGCAACAAC11.0638297872340425No Hit
CTATAGGGATAGTGATCCAGAGCCATAACATGTTTCCATCGCCATCGATC11.0638297872340425No Hit
CTCTCAATATAATCTCGCAGGAGATCATAAGTGCTCCAGGCCTGAGATTG11.0638297872340425No Hit
AGCCAGTAGTCATATGCTTCACTCAAATCATAAGCCATGAATTTCAAACT11.0638297872340425No Hit
CCACCAAACAGCGCCGGGAACAGATAGGCGCTATCGATGTAGGAGCCGAC11.0638297872340425No Hit
CATCTAAGCCCGGCGGATTTGCCTACCAGGCACGACTACAGGCTTGAACC11.0638297872340425No Hit
CCGCCGACGTCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCG11.0638297872340425No Hit
ATCCTGATCCGTGACACCGACGAGGCGCTGGCCAAGCTGCAGGCGCTCGC11.0638297872340425No Hit
CAGCTACTACGGTGGCCAGAAGCAAGACACCCCCAAGGTGGTCAGCTCTG11.0638297872340425No Hit
GATAAAGAAAACGACTGAAGGGGTGAAACTTTACCCTGGAACGCATACAT11.0638297872340425No Hit
AAGTCGAGCCGGTGGGCTGGGGCGGTCAGTGGCTGCGGGTCATGCGCTCG11.0638297872340425No Hit
CACACACCCTCTCTGGCGCACCTATCCAGCGAACTTAGCCCCGGTAAGAC11.0638297872340425No Hit
GGGGGGGGGGGGGTGGGTAATTTGCGCGACTGGTTCCTTCCTTGGAGGTG11.0638297872340425No Hit
AAGCGAAGGTGATGATGCTCTCCATCACTACTCCTTCTATCGATACTAGT11.0638297872340425No Hit
AAAATCAAGAGTCTAGTCTTTCTTTAAGTTCTAAAAATTAAAATTACTAA11.0638297872340425No Hit
GTTGGGAGACGTGTGCTCTTCCGATCTCTGTTGGGAGACGTGTGCTCTTC11.0638297872340425Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTGCGGCACCTCGGTCCTGGCAGCAGCCACCGGCACTGTGACATTCGTTG11.0638297872340425No Hit
ACTTGGAACAGAGGAGTTTGAGATTGCTCCTGAAGACTACATCACCTATG11.0638297872340425No Hit
GCTCTTTTGTCCGGGGACGATAATGACGGTACCGGAAGAATAAGCCCCGG11.0638297872340425No Hit
GCCAAGAGCATGGCGATCGAGCTTGGCCATCCCAACATTCGGGTGCTGGC11.0638297872340425No Hit
GGAGCGGACCGGCTTGGAGAGTATACCCGCGGGCGCCTCGACGACCCGGC11.0638297872340425No Hit
GAGTTGTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCT11.0638297872340425No Hit
CTCTACTTGTGCGCTATAGGTATCTCGCCAATATTTAGCTTTAGATGAAA11.0638297872340425No Hit
GGGTGGCGGTGGCCACCTGCGCGATAGCCTAGAGCGGACTAACGGTGTTG11.0638297872340425No Hit
CTTATACAATGATACAGTCCATGTTGACAATAAGGTCTGCGCACAGCACA11.0638297872340425No Hit
ACGCAGATCCGGCGACACGCCTCCTGAAAGCAGTGTGATACGCGGGAACT11.0638297872340425No Hit
GTCCCCTTTCATGGAAAACCCCGCTCTTTTCAGGACTGGTAACAACGACG11.0638297872340425No Hit
GTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCG11.0638297872340425No Hit
ACTATAAACTTTGCCTTCACATCTCCAGCATTTTCCTTGTTCAGGACACC11.0638297872340425No Hit
TCCGCGGGCTGTCCAGGCTCCCCCTGGTGGACCAGGAGGACTTTGAGAAG11.0638297872340425No Hit
ACCGAATGCTACCGAAGGGTCGGGACTATTCCCACTCGATGGTGCCGGGG11.0638297872340425No Hit
ATCTTCGCCGTCTTCCTGGTCCTGGGCTTTACGGTGGCCAAGAAGGTCAC11.0638297872340425No Hit
AGAAGAGACTAGGACGGTATCTGATCGACTTCGAGCCCCCAACTTTCCAT11.0638297872340425No Hit
TGCCCACCCTGCCCAATCTGAGGGGGCTGGAGCCGCGGGGCGCCCTGTGG11.0638297872340425No Hit
ATCTGAAACTGATCTCCAAAGCTTGAAGAGCTGGTTTGAGAATCGGCTCT11.0638297872340425No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCA50.0145.02
GCAGGGC50.0145.06
AGGGCCC50.0145.08
TAGCAGG50.0145.04
AGCAGGG50.0145.05
AGAGCCC50.0145.0145
CAGGGCC50.0145.07
GAGTAGC50.0145.01
GTAGCAG50.0145.03
GGGCCCG50.0145.09
Produced by FastQC (version 0.11.2)