FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5a11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5a11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692148
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT51370.7421823078301173No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC50930.7358252859215081No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTCC39710.573721227251975No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT36970.5341343180938182No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGGC35490.5127516080375873No Hit
GTCTCGGGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGG32740.47302022110878017No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAG26520.383155047764351No Hit
GGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGGAACTTA19620.2834653860157076No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT18070.26107133156492546No Hit
CCGTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACA16110.23275368851748468No Hit
CCTTAGGACCGTTATAGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAG15470.22350711119587138No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGG15420.22278472234262037No Hit
GACGTATACGGTGTGACGCCTCCCCGGTGCCGGAAGGTTAATTGATGGGG14710.21252680062645563No Hit
GGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATTGT14590.21079306737865314No Hit
CCTTAGGACCGTTATCGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAG14320.20689216757109752No Hit
GTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGG14310.20674768980044733No Hit
GCGTCACACCGTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTT14190.20501395655264482No Hit
TGTTTGCACAGTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATC14050.20299126776354193No Hit
GCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGGAGACAGCCT12570.18160855770731116No Hit
ATTAAAAACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGA12460.18001930223015886No Hit
GGCCATCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATT11770.17005033605529452No Hit
ATACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAACTAGGCAAAATGGTG10930.15791420332067707No Hit
GTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTCCCCGGTGCCGG10820.1563249478435248No Hit
GAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGGAACTTACCCG10700.1545912145957223No Hit
GGTAGAGAATACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAACTAGGCA10550.15242404803596918No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT10520.15199061472401856No Hit
ACCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAA10060.145344637274109No Hit
TCCGTAAGGAGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAC9660.1395655264481007No Hit
GGCTGCAACTGTTTATTAAAAACACAGCACTGTGCAAACACGAAAGTGGA9220.13320850453949154No Hit
CCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGG8760.126562527089582No Hit
CCTTACGGATAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCAC8640.12482879384177951No Hit
CAATTTCACTGAGTCTCGGGTGGAGACAGCCTGGCCATCATTACGCCATT8590.12410640498852846No Hit
CGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGGTT8510.12295058282332681No Hit
GGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATAGT8280.11962759409837204No Hit
CAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGTATACGTCCACTTT8250.1191941607864214No Hit
GGTTAATTGATGGGGTTATCCGTAAGGAGAAGCTCTTGATCGAAGCCCCG8160.11789386085056953No Hit
AGGCAAAATGGTGCCGTAACTTCGGGAGAAGGCACGCTGGCACGTAGGTG7880.11384848327236372No Hit
GAGAATACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAACTAGGCAAAAT7810.11283713887781226No Hit
GTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGGTTATCCGTAA7770.11225922779521141No Hit
TCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGGAGACAGCCTGGC7500.10835832798765582No Hit
CACCGTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAA7490.10821385021700561No Hit
GGTGAAGGAACTAGGCAAAATGGTGCCGTAACTTCGGGAGAAGGCACGCT7470.1079248946757052No Hit
GGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATCGT7410.10705802805180396No Hit
ATCAAGAGCTTCTCCTTACGGATAACCCCATCAATTAACCTTCCGGCACC7390.10676907251050353No Hit
TTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGGAGACAGCCTGGCCA7230.10445742818010022No Hit
CGTATACGGTGTGACGCCTCCCCGGTGCCGGAAGGTTAATTGATGGGGTT7090.10243473939099731No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT14250.091.1079942
CTTCCGA14800.087.722231
TCCGATC15000.086.075293
CCGATCT16100.080.64494
CGATCTA3700.064.693695
TGCTCTT5150.059.150772
GTGCTCT6350.049.1148761
CGATCTG9300.048.35695
CAATAGT604.932147E-448.35694
GCTCTTC6500.047.9849243
CGATCTT6400.045.3345955
TCTAGAC657.317417E-444.637143
CTCTTCC7000.044.557434
TAGACAG700.001053884341.4487725
ATTACAC1401.4073521E-841.4487723
ATCTAGG1053.8247927E-641.4248127
GTATAGA2850.040.7186551
GTTTAGG907.4617616E-540.2945061
CTGTCTA1807.2759576E-1140.279945145
TACACAG2200.039.5647355
Produced by FastQC (version 0.11.2)