FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5a10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5a10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402576
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC8390.20840785342394977No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA6920.17189300902189897No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA6430.15972139422121537No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC6300.1564921902945034No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT6190.15375978697190096No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT6090.1512757839513533No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC5420.13463296371368388No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT5100.12668415404793132No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG4740.1177417431739597No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG4700.11674814196574063No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT4570.11351893803902866No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT4160.1033345256547832No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT4040.10035372203012599No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA5050.057.431791
TTCCGAT5200.055.7751122
CCGATCT5500.051.414514
TACACTA951.9166127E-645.7943045
CCGTGTA657.336508E-444.6099
CGATCTA1851.8189894E-1243.112655
TCCCCCC3400.042.6409578
TATACCG907.469821E-540.282025
CCCCCCG3600.040.2720159
ACAGGTA1157.139093E-637.820688
GTATAAC1354.9574555E-737.5965541
ACTCCCC4000.036.253826
CCATACT4200.034.5274472
CGTATAC850.002738632534.1212433
CTCCCCC4050.034.007487
ATACTCC4300.033.7244834
CAGGTAT1551.4637626E-632.737259
GTAGCGT900.003623805232.2256166
CATACTC4650.031.1860833
ATACCCG3300.030.7608175
Produced by FastQC (version 0.11.2)