FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4h9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4h9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448583
Sequences flagged as poor quality0
Sequence length151
%GC57

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG5310.11837274261396442No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC5300.11814981842825074No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG4520.10076173194258364No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGC400.006043125454.3877143
TTATACC1601.0059011E-940.786236145
CTTCCGA9950.040.089221
TTCCGAT10000.039.8843232
GTCTAAC750.001479999238.680021
GTGTACT800.002031971736.262524
ATTATAC1051.8495473E-434.531883
CCCTAGA850.002737240934.1256262
AGTAGTA1102.4306786E-432.9622542
TACAATC1102.4306786E-432.9622542
TAAGCCT1402.7368604E-531.0821594
TATATAA1652.378214E-630.7647672
TCCGATC13350.029.8758963
GTCTACG3201.8189894E-1229.4632991
TATATTG1000.006060696729.0100175
TCTAAGC1000.006064000529.006782
TACTGCT1255.1524E-428.997087
CCGATCT14700.028.1219544
CATAATC1306.46848E-427.8911363
TATACGG1050.00769520827.6255052
Produced by FastQC (version 0.11.2)