FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4h8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4h8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541730
Sequences flagged as poor quality0
Sequence length151
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT18050.035.7634542
CTTCCGA18100.035.2704471
TATACAG1458.6591535E-735.0152055
CCGATCT19900.032.438714
TCCGATC20300.031.7995223
AGTATAG1402.7362392E-531.0849255
CTAGCTG1000.006059160529.0125963
GTATAGA2104.9524715E-727.636151
CGATCTT9550.027.3417155
TATATAC1606.802309E-527.1993083
ATAGAGG2207.4470336E-726.3750883
TCTACAC2051.2845125E-524.766853
ACTGTGC2051.2900404E-524.7531348
ATACAGT2451.9021863E-623.6837546
CATACTC2502.267434E-623.2100773
GTAATAT1952.6049325E-422.317383
GTGCTCT15400.022.1403241
TAGAGGA2303.1280862E-522.0748024
TCTATAC2303.1280862E-522.0748023
TCTTCCG15150.022.0227645
Produced by FastQC (version 0.11.2)