FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4h6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4h6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences792912
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA16670.2102377060758319No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC16080.2027967794660694No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG13230.16685332041891154No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA13050.16458320721593317No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT12430.15676392840567427No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC12240.1543676978025304No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT11320.14276489698730754No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT10280.12964868737009907No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT10200.12863974816877535No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC10190.1285136307686099No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA9060.11426236454991222No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT8980.1132534253485885No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT8340.10518191173799867No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT8240.10392073773634401No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG8050.10152450713320016No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGGG1353.6379788E-1253.716793
CCGATCT9850.050.7991754
TTCCGAT10700.046.760782
CTTCCGA11300.043.64171
TCCCCCC7650.033.1675538
ATACTCC8150.032.922134
CGATCTG6100.032.0979845
CCATACT8450.030.8931482
CCCCCCG8500.029.8507989
GTATAAA2701.70985E-1029.5460991
ACTCCCC8600.029.5130066
TATAGGT6400.029.46033
CTCCCCC8950.029.159927
GTCGCGC6400.028.318288
ACCATAC9500.027.4821111
GGGCGAC3204.1836756E-1127.1855497
GGTCGCG6700.027.0502997
TCGCGCG6700.027.0502999
CATACTC9700.026.9137733
TCTTCCG6800.026.6609085
Produced by FastQC (version 0.11.2)