FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4h5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4h5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences979629
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC15910.16240842196382507No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA13180.13454072919441953No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA13130.13403033189095054No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT12020.12269951175393949No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG11920.12167871714700157No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC11180.11412483705566087No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT10630.10851046671750224No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC9870.10075242770477394No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCCCC6300.040.2742468
CCCCCCG6750.038.6632779
CCGATCT11800.036.2542274
CTTCCGA12950.033.5946461
TTCCGAT12600.033.3769072
CTCCCCC7900.033.0350767
ACTCCCC8300.032.3230446
ATACTCC8700.030.8369264
CCATACT9050.030.4455362
GTATATA5200.029.2822591
CGATCTG6700.029.2198225
CATACTC11050.028.8721453
CGAGTCG7700.028.2500466
GTCTACG10200.027.7238181
ACCATAC9500.027.4768851
ACACATA7200.027.192055145
TACTCCC10350.026.6214625
GTCGGGT8400.025.8905879
TACGAGT8800.025.5427474
TCTACGA11200.025.2484782