FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4h3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4h3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1136092
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA16420.14453054858233313No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC15230.1340560447569387No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA13060.11495547895769005No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC12950.1139872475116452No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT11700.10298461744295355No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA15500.043.9734041
CCGATCT17550.040.90634
TTCCGAT18200.038.246682
TCGCGCG6150.033.008029
CGATCTG10050.031.7481755
GTATATA7000.030.0395811
TATAGGT7300.028.8076133
GTCGCGC7100.028.5914548
GGGCGAC5350.028.4578487
GGTCGCG8100.025.9567057
AGGTCGC7850.025.8654796
TAGGACA4250.025.5937824
CGATCTA4750.024.4263465
CTATATA4201.8189894E-1224.1697771
TCCGATC30700.023.6207563
CCTACAT3402.4992914E-923.4609683
CGAGTCG8500.022.1812786
TACGAGT9350.021.7159374
TTAGGAC5151.8189894E-1221.1210843
GGCGACC6900.021.0144238
Produced by FastQC (version 0.11.2)