FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4h2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4h2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1106370
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC16030.144888238112024No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA15120.13666314162531523No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA12760.11533212216527924No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC11220.10141272811084899No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA17950.043.226881
CCGATCT18400.042.5580374
TTCCGAT18400.041.7718122
TCGCGCG6300.034.5199
GTCGCGC6300.033.3683668
CGATCTG9850.032.388595
TCCCCCC6500.032.341658
CTCCCCC7100.030.6295367
CCCCCCG6950.030.2475859
TATAGGT8000.029.9088423
ATAATAC4900.029.59443
GGTCGCG7250.028.9959587
TAAGGTG9200.026.79585
AGGTCGC7850.026.7857636
TCCGATC30650.026.2583923
CGATCTA6050.025.1674695
AGGTGCC8700.024.9965177
TGTACCG3902.0008883E-1124.1687625
ACTCCCC9400.023.9116466
CCATACT10050.023.8091092
Produced by FastQC (version 0.11.2)