FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4h1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4h1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435688
Sequences flagged as poor quality0
Sequence length151
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA11770.27014744496061405No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC10360.23778483685573162No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG9350.21460311048273076No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA9040.20748792714052258No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT8750.20083178788490846No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA8510.19532325884577956No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT8280.19004425184994767No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG8220.18866711959016544No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC8000.1836176346376306No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC6540.150107416316263No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG6450.14804171792658968No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA6310.14482840932043115No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT6050.13886083619470815No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT5990.13748370393492593No Hit
CTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGG5320.12210572703402435No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG5070.1163676759515984No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA4730.10856392647949908No Hit
CATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATG4680.10741631626301389No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCG4380.10053065496410274No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA3900.050.195135
TTCCGAT21300.044.9321022
CTTCCGA21500.044.8565061
GTATAAG657.3258334E-444.6230161
CCGATCT21450.043.9418644
TACTAAC1903.6379788E-1241.980861
GATCTAG1903.6379788E-1241.9760446
CTAACAC2400.039.273043
ATCTAAC750.001482893138.6644027
CTACTTG2450.038.471553
CTCTACT2201.6370905E-1136.2562031
ATCTAGC1209.555306E-636.2478757
TCCGATC26750.035.777713
TCTACTT2302.5465852E-1134.675862
GTATGGG1051.8501433E-434.5297131
GTCCTAA2404.3655746E-1133.2348481
TATACGG900.003625003232.2240332
ACACATA2051.1335942E-831.834713145
TACACTT950.004723099530.5280325
CGATCTC8400.030.210035
Produced by FastQC (version 0.11.2)