FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4h12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4h12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences313095
Sequences flagged as poor quality0
Sequence length151
%GC56

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACTG1053.8092403E-641.438755
CTAACGT1651.3569661E-939.5551723
TTCCGAT7150.036.5124662
TAGAGTG1406.5776294E-736.2589075
ACTATAG800.002032029236.2589073
TAGGGTG800.002032029236.2589075
ATAAGAC800.002032029236.2589073
CTTCCGA7350.035.5189321
TTAGAGT1458.6563887E-735.0086024
ATACAGT1051.8481443E-434.532296
GCCTAGG850.002735823634.126031
TAACGTT2008.887582E-932.633024
CCTAACG2008.887582E-932.633022
CTCATAA1801.2848068E-732.230141
TAGGGTT900.003620094232.230144
CTATGAG1402.7361026E-531.0790653
ATAATAG1402.7361026E-531.0790653
TATGAGT950.00471671530.5338174
CCCTAAC2151.7977072E-830.3562951
CTGTACA1204.053495E-430.2061049
Produced by FastQC (version 0.11.2)