FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4h11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4h11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences564726
Sequences flagged as poor quality0
Sequence length151
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG16320.2889897047417686No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG14010.2480849119750109No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC10710.1896494937367856No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC9650.1708793290905678No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC8100.14343239022109838No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA7430.131568229548489No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT7030.12448514854991626No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC6810.12058945400070123No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC6720.11899576077602236No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA6590.1166937594514862No Hit
GGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGCT6560.11616252837659326No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCT6520.11545422027673596No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG6510.11527714325177167No Hit
CCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCA6310.11173560275248527No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA6230.11031898655277073No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCG5870.10394421365405523No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA5870.10394421365405523No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAAT5780.10235052042937637No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAC604.928708E-448.362196
TTCCGAT18550.046.1420442
GATCTAG951.9115141E-645.8168146
CTTCCGA18600.045.6280251
CCGATCT21400.040.3394934
TAGATAG1303.6773054E-739.061775
TCCGATC23500.037.3520773
GTCTAGT800.002030791736.268431
CTAGAGA950.004711792330.5445424
CGATCTG13250.029.0173155
TAGAGTG4050.028.6590795
TAGAACA1804.673444E-628.2112794
TATACAG1555.47393E-528.081275
ACCTATA11450.027.8744281
CCTACAC2354.265894E-827.7825363
GTACTAC2104.957892E-727.633091
TTAGAGT4600.026.8094754
CTAACGT9000.026.5992073
CCTAACG9550.026.5841882
ATCCTAC2207.4431773E-726.377041
Produced by FastQC (version 0.11.2)