FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4h10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4h10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483780
Sequences flagged as poor quality0
Sequence length151
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG21000.4340816073421803No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC12290.2540410930588284No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG11470.23709123981975277No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC10090.20856587705155236No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA9860.20381164992351897No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC8480.17528628715531852No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG8310.17177229319111995No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC8130.16805159369961553No Hit
CTATCAGACGGGTTTCCCCCGACTCTTAGGATCGACTAACCCATGTGCAA6360.1314647153664889No Hit
CCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCA6350.1312580098391831No Hit
ATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAAC5950.12298978874695109No Hit
ACCTCCGGCTGAGCCGTTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGA5860.1211294390011989No Hit
ATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGG5830.12050932241928149No Hit
GATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGAAG5700.1178221505643061No Hit
ATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCGCTTAAGCGCGAACTTC5680.11740873950969448No Hit
ACCTAGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGTGCAGATCTTGGTGG5670.11720203398238868No Hit
ATTTTAACCGGATCCCCTTTCGAAGTTCGCGCTTAAGCGCTATCAGACGG5650.1167886229277771No Hit
CTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTT5430.11224110132704948No Hit
GCACATGGGTTAGTCGATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCG5180.1070734631444045No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA15900.059.7599681
TTCCGAT16100.059.4681242
CGATCTA3050.052.318955
CCGATCT18400.052.0346074
TCCGATC19700.048.6008533
ATACCGA700.001053730741.447486
CGATCTG9050.038.4705925
GTCTAGG951.0253443E-438.1753121
GAGTTTA2206.184564E-1032.969591
TTAGACT1204.0441498E-430.2221224
GTGCTCT10600.029.4238011
CGATCTT7900.029.3804935
GTCTAAT1000.00605787629.0132371
GATCTGA3750.029.0132356
GTGTAAG1804.676738E-628.2073141
GATCTAG1804.676738E-628.2073141
CTAATCC1555.477573E-528.0773283
TTAGTAC1306.4608094E-427.8973433
TCTTCCG11250.027.7237595
CTAGGGG1050.0076874427.6316552
Produced by FastQC (version 0.11.2)