FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4g8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4g8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1455639
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC21790.14969370839885437No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA21620.1485258364196068No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC17830.12248916111755731No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA16750.11506973913174902No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT16000.10991736275271548No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT15590.10710073033217715No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG14910.10242924241518672No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA21250.044.3653641
CCGATCT21600.044.3179664
TTCCGAT23750.040.3046382
CGATCTG13450.035.0469935
TCCCCCC8600.031.188818
GTCGCGC8400.031.0683928
TCGCGCG9100.028.6785149
GGTCGCG9500.027.4709957
TATAGGT10150.026.4359763
AGGTCGC10150.026.4359766
TATACAG6650.026.1727435
CCCCCCG10000.026.0974469
CTCCCCC10850.024.7210857
TCCGATC41200.023.4106833
ACTCCCC11800.023.3539986
CTAGGGG2903.2793287E-722.5063043
CCATACT13600.022.3952082
TCTTCCG15650.022.2426515
TAAGGTG12950.021.8400945
ATACTCC13300.021.810624
Produced by FastQC (version 0.11.2)