FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4g7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4g7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464086
Sequences flagged as poor quality0
Sequence length151
%GC57

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG6090.13122567799933632No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAAG350.00356463862.1457671
GTATATG701.694095E-551.788141
CTAGACT450.00963412448.3355984
TTACACT657.330812E-444.6174744
TAGACTG855.335577E-542.6490555
GTATTAG700.001055811941.430511
TCTAGAC907.47417E-540.2796633
TTCCGAT11700.039.040292
CTTCCGA11900.037.774881
ATTATAG1354.961912E-737.5943533
TAGTACT1051.8516796E-434.5254254
TATTAGC850.002739849334.1192442
TCCGATC14250.033.580523
CCTAAGG1102.4334785E-432.956092
GTCCTAA900.003625411532.2237321
TACACTG900.003625411532.2237325
CATATAA1601.8735645E-631.7202341
ATTATAC1601.8735645E-631.7202343
TATAGGC950.004723630430.5277442
CCGATCT15700.030.4791324
Produced by FastQC (version 0.11.2)