FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4g6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4g6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences989994
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA15150.034.4635281
CCGATCT15550.034.0450744
TTCCGAT17700.030.3163132
TCCGATC23800.023.7672773
CGATCTA4950.023.4410235
TAGTACT5950.023.157864
TATAATG3604.836693E-922.1568532
ATCTAGG2353.702428E-521.600811
GTCTATA3055.32802E-721.3984581
GTACTAA2806.07782E-620.7191431
TATAGTA3157.2687544E-720.7180962
TCCCCCC5950.020.7139118
ACAATAC8800.020.6024633
TATAGGT4952.1827873E-1120.5108953
TATAGTG2857.08036E-620.3566785
TAATAGT3601.1725024E-720.144634
CCTACAC5453.6379788E-1219.9598163
TATACAG4402.2555469E-919.7783645
GTATATA6600.019.7773651
CCTATAA9800.019.2392041
Produced by FastQC (version 0.11.2)