FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4g5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4g5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642176
Sequences flagged as poor quality0
Sequence length151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG26430.41156941399242575No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC15780.24572702810444486No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA12650.19698649591389275No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC12180.18966763005780346No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC12180.18966763005780346No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC11660.1815701614510664No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG10480.16319513653577836No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG10080.15696631453059598No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA9630.1499588897747658No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT9270.14435294997010165No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA9180.1429514650189356No Hit
ACCTCCGGCTGAGCCGTTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGA8100.1261336456049432No Hit
ACCTAGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGTGCAGATCTTGGTGG7730.1203719852501495No Hit
ATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGG7400.11523320709587402No Hit
GATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGAAG7140.11118447279250547No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC6780.10557853298784135No Hit
ATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAAC6760.10526709188758221No Hit
CTATCAGACGGGTTTCCCCCGACTCTTAGGATCGACTAACCCATGTGCAA6460.10059547538369543No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT6430.10012831373330676No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA25250.066.3364941
TTCCGAT26550.063.6345982
CCGATCT28700.058.35774
TCCGATC33950.049.9740073
CGATCTA5950.048.7428745
CGATCTG14250.046.810265
TCTAGGG855.3347158E-542.6533362
ATCTGTG1859.640644E-1139.18587
TCTAGAC800.00203460336.2553332
TAGACGT2201.6370905E-1136.2525144
CATACTC4150.034.942183
CCATACT4300.033.725892
ATCTGCG3550.032.6732337
CGATCTT10350.032.2244575
ATACTCC5000.031.902214
CGATCTC12350.031.1155155
CTATGCT950.00472804930.5236769
ACCATAC4650.028.0686451
ATCTATG1050.00770089727.6231142
TCTTCCG15550.027.0436735
Produced by FastQC (version 0.11.2)