FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4g4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4g4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318626
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG5760.1807762078424234No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC4160.13056059455286137No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA3900.12240055739330752No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC3880.12177286222718799No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA3740.1173789960643513No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3310.1038835499927815No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA400.006046054454.377411
CTTCCGA17500.045.5734561
TTCCGAT17750.045.340042
CCGATCT18250.043.303294
ATGTAGG855.3318705E-542.648951
TACGCTA700.001055325341.430415
TCCGATC19700.040.8520663
TATAGCC750.001481544138.6683852
GCAATAC800.002034092636.251616
ACCTATA1655.992479E-835.1530761
ATTAGGG1051.8504054E-434.5253453
CGATCTT6850.032.8116765
TTAGGGC900.003623759832.223654
TCTAGCC900.003623759832.223653
TTAAGGT1153.1564775E-431.5231384
CTTCTAG1153.1564775E-431.5231381
GATCTAG1402.7400196E-531.0728071
CGATCTG10050.031.0212785
TTCTAGC950.00472148530.527672
CTAACAT950.00472148530.527673
Produced by FastQC (version 0.11.2)