FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4g3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4g3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences637749
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG14010.21967890188773326No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC8990.14096454874880243No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC8550.1340652827366252No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT8470.13281087073441117No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA7300.11446509520203091No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC6790.1064682186879164No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA32850.052.778281
TTCCGAT34000.051.419852
CCGATCT36900.047.178434
TCCGATC38600.045.664383
TAGAAGT803.7219383E-545.335524
CGATCTA6700.042.222935
TAGCACT2500.040.620634
CTATAGG907.453577E-540.3014031
TCTAGAC1458.6520595E-735.020532
GTATACG850.002733373334.1376571
CTAGACG1301.6481044E-533.478543
ATAGCAC2603.6379788E-1233.478543
CGATCTT14750.032.457165
CGATCTG20100.031.3965425
TATAGTG1652.3752073E-630.7732055
GATCTAG3251.8189894E-1229.0170126
GTCTAAG1000.00605545429.017011
GTATATG1000.00605545429.017011
TGCTAGG1000.00605545429.017012
ATCTAGG1555.4754142E-528.0809781
Produced by FastQC (version 0.11.2)