FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4g2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4g2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences600366
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG38300.6379441873790321No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG25810.4299044249674365No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC22620.37677017019618037No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA19100.31813926837962175No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC18090.30131619711975693No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC16620.2768311330088646No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC15970.2660044039802387No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG15390.25634363038546487No Hit
GGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGCT12520.20853945759753217No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCT12330.20537472141993382No Hit
CCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCA11320.18855165016006903No Hit
ACCTCCGGCTGAGCCGTTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGA10680.17789148619342202No Hit
ACCTAGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGTGCAGATCTTGGTGG10310.1717285789002042No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAAT10170.16939666803250017No Hit
ATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGG9920.16523254148302868No Hit
GATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGAAG9920.16523254148302868No Hit
CTATCAGACGGGTTTCCCCCGACTCTTAGGATCGACTAACCCATGTGCAA9860.16423315111115552No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC9570.1594027643137686No Hit
CTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTT9330.15540520282627598No Hit
ATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAAC9130.15207390158669878No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT9100.1515742064007622No Hit
ATTTTAACCGGATCCCCTTTCGAAGTTCGCGCTTAAGCGCTATCAGACGG8890.14807634009920614No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT8820.14691038466535414No Hit
AGCCCGGGCGGAGCGGCCGTCGGTGCAGATCTTGGTGGTAGTAGCAAATA8740.14557786416952326No Hit
ATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCGCTTAAGCGCGAACTTC8440.14058091231015749No Hit
AGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGC8290.13808243638047457No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCG8220.13691648094662257No Hit
TTGTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCT8030.13375174476902424No Hit
GATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCTAGGTTTTGCAG7910.13175296402527792No Hit
CTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCG7790.1297541832815316No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTA7610.12675601216591212No Hit
GTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTT7570.12608975191799668No Hit
CTTAAGCGCTATCAGACGGGTTTCCCCCGACTCTTAGGATCGACTAACCC7410.12342471092633495No Hit
AGATAACTCTTCCCGAGGCCCCCGCCGACGTCTCCGGACTCCCTAACGTT7200.11992684462477889No Hit
GCACATGGGTTAGTCGATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCG7100.11826119400499029No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA7030.11709523857113828No Hit
GGTTAGTCGATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCGCTTAAGC7010.11676210844718056No Hit
GTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCG6960.11592928313728626No Hit
CTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACC6880.11459676264145538No Hit
GTTAAACAGAAAAGATAACTCTTCCCGAGGCCCCCGCCGACGTCTCCGGA6810.11343080720760336No Hit
CTGTTAAACAGAAAAGATAACTCTTCCCGAGGCCCCCGCCGACGTCTCCG6740.11226485177375134No Hit
GTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGT6650.1107657662159416No Hit
CTGCAAAACCTAGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGTGCAGATC6630.11043263609198388No Hit
GTCTTAATCGACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGG6510.10843385534823759No Hit
ATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGCGCG6500.10826729028625873No Hit
CATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTG6270.10443629386074495No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA6180.10293720830293521No Hit
GATTAAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGAGT6080.10127155768314662No Hit
CGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCG6050.10077186249721004No Hit
GCGCTTAAGCGCGCGACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCT6040.10060529743523118No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA23500.069.117061
TTCCGAT24300.067.731162
CCGATCT29050.056.6563574
CTAGACT553.2168324E-452.731034
TCCGATC34250.048.266213
TCTAGCG657.331658E-444.618563
CGATCTG13950.044.1787725
CGATCTA7150.043.6045075
TATATCG700.00105593341.4315265
ATCTGAG1701.8262654E-938.381897
ACACATA1951.70985E-1037.17904145
TGCTCTT12550.036.9747472
GATCTGA4650.035.862776
GATCTAC3100.035.0831456
TCTTCCG14000.034.1810045
CGATCTC9400.033.9385875
TAGACTG1352.1397418E-532.2245185
CTATCAT3000.031.4189033
GGCCCTA2601.10958354E-1030.6727077
GTGCTCT15700.030.4824181
Produced by FastQC (version 0.11.2)