FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4g1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4g1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238107
Sequences flagged as poor quality0
Sequence length151
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3890.16337192942668632No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2920.12263394188327098No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTATA451.18911135E-464.476011
TCTAGGG400.00603354354.4016342
TAGGGAC400.00603354354.4016344
GGTTTAT604.9255276E-448.3570061
TAGACGT1002.7161568E-643.521314
GTATTAA1252.6969064E-740.619891
GAGTACT900.003614042432.2380071
CTAGATG950.00470884330.541274
CCCTAAC950.00470884330.541271
TGTAGAC1204.03725E-430.223132
GTATTAT2202.2382665E-829.673621
CTTTAAC1000.0060507929.0142062
CTTCCGA10150.028.5854241
TTCCGAT10250.028.3065412
CCGATCT10950.027.1594144
ATATTAC1658.364227E-526.376553
TCCGATC11300.025.6762873
ACACATA2101.5402362E-524.183586145
TTAAACA1801.5105146E-424.1785034
GGACTCC1851.8189177E-423.5250326
Produced by FastQC (version 0.11.2)