FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4g12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4g12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences972069
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC12500.12859169462250106No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA10770.11079460408674692No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT11450.031.0386984
CTTCCGA12450.027.3790951
CTAACGT5200.026.5010573
TCCCCCC6050.026.3634038
CCCTAAC6150.025.944071
GTATATA5600.025.9019571
TTCCGAT13400.025.4380382
CCATACT8450.024.8903982
CCTACAT3753.0559022E-1023.2093453
CTCCCCC7400.022.5335857
CGATCTG7250.022.0088635
ATACTAC4950.021.9785463
CCTACAC5001.8189894E-1221.758763
CCCCCCG7350.021.7004899
GACTTAG5051.8189894E-1221.542221
TAGACAG9100.021.5196555
TCTAGAC4109.349606E-1021.228063
ACTCCCC7900.021.1160966
CTAAGAC3809.00036E-920.9952963
TATACTG4851.6370905E-1120.9362645
Produced by FastQC (version 0.11.2)