FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4g11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4g11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1216409
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC33520.2755652087414677No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA32980.2711259124192603No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA28180.2316655006663055No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT25210.2072493708941647No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC24310.19985054369048566No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG23460.1928627624425666No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT22530.1852173076654316No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT19720.16211652495172266No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT18870.15512874370380356No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC17870.14690782458860466No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT17550.144277130471741No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA17060.14024888010529352No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT17010.1398378341495336No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA16470.13539853782732617No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT15600.12824633819710313No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA15210.12504017974217552No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG14250.11714809739158456No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA13910.11435298489241694No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC13280.10917380584984163No Hit
GTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGA13140.10802287717371377No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG13080.10752962202680183No Hit
ACACAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAG13000.10687194849758593No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA12550.1031725348957464No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA14250.042.7446981
TTCCGAT14400.042.29772
CCGATCT15400.040.4977844
CATACTC12450.037.278993
ATACTCC12200.036.854064
TCCCCCC12250.036.6945848
CCATACT13300.034.8921932
GGGCGAC5850.033.4621857
ACCATAC12750.032.9864541
TCGCGCG9500.032.816429
CTCCCCC14000.032.6256267
CCCCCCG14000.032.107769
TATAGGT10300.030.9791133
CCTACAC10450.029.8404733
GTCTACG12650.029.8078461
GTCGCGC10750.029.6749928
ACGAGTC11800.029.499375
GGTCGCG10650.029.2728657
TACGAGT12400.029.241654
TCCTACA10500.029.0041352
Produced by FastQC (version 0.11.2)