FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4g10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4g10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1191384
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA17310.14529320521343245No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC16020.1344654620172841No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA13680.11482443947543361No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG13000.10911679189916935No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT12350.1036609523042109No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT13150.039.1515734
TTCCGAT14000.036.7729642
CTTCCGA14250.036.1278271
CCCCCCG8850.033.585199
TCCCCCC9050.032.842978
CTCCCCC10300.028.8571767
CCATACT11750.027.7698152
ACTCCCC11800.027.0387926
CGATCTG10100.026.5642135
ATACTCC12000.025.9838684
ACCATAC12400.025.7293821
ACTACCG5650.025.6683625
CATACTC12750.025.0241343
CCGTGGG6350.022.8330259
TCTAGGC2953.870482E-722.121722
CCTATAT2953.870482E-722.121721
TACTCCC14500.022.003985
GTATTAT4659.094947E-1221.830991
AGGTCGC6000.021.7539356
CATACTA6000.021.7530232
Produced by FastQC (version 0.11.2)