FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1921443
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA47520.24731412797569327No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC46150.24018406999322905No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA37900.19724758944189338No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC36300.18892051442587682No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT34140.17767896315425438No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT33810.17596150393220095No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG33440.17403586783474712No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT31620.16456382000402822No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA26300.136876295575773No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT25790.1342220404144177No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC25490.1326607138489146No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT25140.13083916618916094No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT25050.13037076821951002No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT23920.1244897714894483No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA23080.12011805710603958No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA22670.11798424413318531No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA21240.11054192083762047No Hit
CTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGG20620.10731517926891405No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC20160.10492114520180926No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG20070.10445274723215833No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA19800.10304755332320552No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT24550.056.705532
CTTCCGA26150.053.237361
CCGATCT28150.050.2289924
CGATCTG15250.038.513545
TCCCCCC18850.029.999488
TCGCGCG15300.029.852429
GTCGCGC15350.029.7551848
TATAGGT16350.029.2701173
ACCATAC20100.029.2197131
ATACTCC21800.028.6048874
CGATCTA7450.028.2253915
GGTCGCG16400.027.8501247
CCATACT22300.027.3117852
AGGTCGC17050.026.793276
ACTACCG6400.026.0583045
CATACTC24050.025.9287553
CCCCCCG21850.025.8805589
CCTACAT9350.025.5917853
CTCCCCC22100.025.5877887
GGGCGAC10250.025.462977
Produced by FastQC (version 0.11.2)