FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408997
Sequences flagged as poor quality0
Sequence length151
%GC56

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGGCCCAAGCGACTGTTTATCAAAAACACAGGTCTCTGCTAAACCG7670.18753193788707498No Hit
CGTTGAGACAGTGCCCAAATCGTTACGCCTTTCGTGCGGGTCGGAACTTA6780.16577138707618883No Hit
GTATAGGGGCTGACGCCTGCCCGGTGCTGGAAGGTTAAGAGGAGTGGTTA5520.13496431514167584No Hit
ATTCTAAGGTGAGCGAGCGAACTCTCGTTAAGGAACTCGGCAAAATGACC4300.10513524549079821No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT11700.050.2008172
GTACATA604.9375003E-448.341531
TAGACTG604.9375003E-448.341535
CTTCCGA12350.047.558671
TCCGATC12900.046.0930823
GTATAGC1401.4088073E-841.4355931
AATACAG700.001055027641.4355935
CCGATCT14250.041.2175144
ACTGTAC1105.2505493E-639.552163
TATAGCG750.001481125538.673222
GTATACG800.00203351736.2561451
TTATACC1655.9930244E-835.157475145
GTCTAAT1051.8499607E-434.5296631
GTATAGA1301.6504833E-533.4672131
CATACTG1551.4602665E-632.7474865
CATTATG900.00362273432.2276842
TTATACT900.00362273432.2276843
CGATCTA2950.031.9545675
TATAGAG1153.1557167E-431.5270822
ATTACAT1402.7395648E-531.0766943
Produced by FastQC (version 0.11.2)