FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444007
Sequences flagged as poor quality0
Sequence length151
%GC56

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGGA604.934958E-448.347493
CTTCCGA15850.040.72171
TATATAG907.462816E-540.2895743
TTCCGAT16200.039.841912
CCATACT2750.039.5570372
TAGACCC1303.6837082E-739.0498965
ACCATAC3200.036.260621
TAGACGT1803.1814125E-936.2606164
CCGATCT17900.035.6528974
TATACTT850.00273641934.127645
ATTAGCC1301.6494081E-533.471343
CATACTC3300.032.9641953
GCTAGAC900.003620878332.231663
CTCTAGT900.003620878332.231661
GATCTTT5200.032.0767026
TCCGATC20000.031.9093443
CTAGACA1153.1538663E-431.5309734
GTCTTAG1153.1538663E-431.5309731
TTAATGT950.004717730530.535264
CAGTACA1204.047496E-430.217184
Produced by FastQC (version 0.11.2)