FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences684186
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC10750.15712101679952525No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA8720.1274507224643591No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA8120.11868117734066468No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG7540.11020395038776007No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT7520.10991163221697024No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC7150.10450374605735865No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA6900.039.9281581
TCCCCCC5000.039.147758
CCCCCCG5250.037.2835739
CCGATCT7450.036.0072754
TCGCGCG3550.034.7163169
GTCGCGC3600.034.2341468
CTCCCCC5900.033.176067
TTCCGAT8150.032.9146232
ATACTCC7050.032.9083144
GGTCGCG4050.032.2203677
ACTCCCC6150.031.8297656
TAGGCAG1601.8757419E-631.7192485
CATACTC7950.031.0067733
ACCATAC6800.030.91961
CCATACT7300.030.788152
TACTCCC7500.029.9671345
TATAGGT4650.028.0649553
GTATAAA3403.6379788E-1227.7210221
TGTAGGC3453.6379788E-1227.3192652
TATACTG3204.1836756E-1127.1879255
Produced by FastQC (version 0.11.2)