FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508656
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC8320.16356830549526596No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA7910.15550784813311944No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA6470.12719794910509263No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC6250.12287282564247744No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG5900.11599194740649868No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT5730.11264980654902332No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT5680.1116668239438835No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGC3300.039.5412458
TTCCGAT7550.039.386032
CCGATCT7350.038.4842074
GGTCGCG3400.038.3782657
AGGTCGC3250.037.9376876
CTTCCGA7950.037.4043431
TCGCGCG3500.037.2817469
TATAGGT3600.036.2639663
TCCCCCC2900.034.9962778
CCCCCCG3250.031.2274469
TAGCACA1652.376235E-630.7694244
GTTCGTA1652.376235E-630.7694241
GTCTAGG1204.046127E-430.2199731
TAAGTGT1952.594279E-729.7550474
CCTACAC3450.029.4316253
GTAAGTG1504.3829787E-529.0111713
TAATACT3403.6379788E-1227.7312664
GTGTTAG1358.0590666E-426.8621961
TATACCG1358.0590666E-426.8621965
GTGCAGG1658.388577E-526.3737931
Produced by FastQC (version 0.11.2)