FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences750202
Sequences flagged as poor quality0
Sequence length151
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT25100.064.129342
CTTCCGA27150.059.5621641
TCCGATC27350.058.3234063
CCGATCT27400.057.952364
CGATCTA5250.044.1944965
TGCTCTT14950.037.3445362
CGATCTG15600.035.7885135
GTGCTCT15850.035.2287251
CGATCTC12800.033.421015
GATCTAG2900.032.502966
ATCTTAC1902.077395E-730.5229877
CGATCTT12950.029.6745175
TCTTCCG19000.029.0026425
GCTCTTC19250.028.6259843
TAGAACG1358.076525E-426.8542964
TCTAGAC2705.5533746E-926.8542962
GATCTTT9350.026.3660356
ATCTAGC2207.4889067E-726.3607647
AATACTA2808.181814E-925.8952145
GATCTTA2559.471296E-825.5905656
Produced by FastQC (version 0.11.2)