FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598427
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG17450.29159780558029635No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG16270.2718794439422018No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC13060.21823881609619886No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC10750.17963761661823416No Hit
GGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGCT8790.14688508372783982No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA8390.14020089334204508No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC8090.135187750552699No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG7900.1320127601194465No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC7890.1318456553598016No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCG7870.1315114458405119No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT7690.12850356016690423No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCG7470.12482725545471711No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCT7450.12449304593542738No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA7350.1228219983389787No Hit
GTCTTAATCGACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGG7070.11814306506892236No Hit
GATTAGAGGCATTGGGGGCGCAACGCCCTCGACCTATTCTCAAACTTTAA7020.11730754127069801No Hit
TTGTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCT6790.11346413179886602No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT6700.1119601889620622No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGC6540.10928651280774432No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAAT6450.10778256997094049No Hit
CCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCA6330.10577731285520205No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT6260.10460757953768797No Hit
CTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCG6120.10226811290265982No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC6070.10143258910443545No Hit
GTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGT6070.10143258910443545No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA21900.044.6990241
TTCCGAT21900.044.367922
CCGATCT22600.042.355534
TCCGATC26200.037.3660473
CGATCTA3750.034.8085445
TATAGGT10100.030.8739193
GTATAAG950.00472205230.5312611
CGATCTG11900.029.860275
TGCTCTT15450.029.5678962
GTGCTCT15800.029.3718451
ATCTTAC1000.00607268828.999857
TCTTCCG16650.028.7457965
TATACTG1306.470585E-427.8914645
CGATCTT10050.027.4196685
TTATCCT2207.461513E-726.370114
TAGGCAG1409.975584E-425.8992145
TAGACGT2259.077794E-725.7841074
GAGTTTA3108.54925E-1025.7299731
GTGTAGA2001.06319985E-525.379111
GTGTAAG1450.001224710325.004051
Produced by FastQC (version 0.11.2)