FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4f1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4f1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739378
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC9840.13308483617310768No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA9380.12686339057964938No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA8670.11726072455496377No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCCCC5200.034.8513648
TCGCGCG3350.034.622499
CTTCCGA6750.033.310111
CCCCCCG5450.033.2526749
ACCATAC5700.033.0838971
GTCGCGC3750.032.862518
CCGATCT7300.032.7875674
AGGTCGC4100.031.8424746
GGTCGCG3650.031.7768027
CCATACT6450.031.4837952
CCTACAC4850.031.4047783
CTCCCCC5850.030.978997
TAGGGTG1753.760697E-629.0120325
TTCCGAT8000.029.0100692
ATACTCC7450.028.2331834
TACTCCC7900.027.543075
TCCTACA5300.027.3679892
ACTGCGT1606.831646E-527.1840638
TATAGGT4850.026.918383
TAGGTCG5500.026.3745755
Produced by FastQC (version 0.11.2)