FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4f12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4f12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645956
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC12310.19057025555920218No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA10850.16796809689824077No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA10130.15682182687365703No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG9420.1458303661549703No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC9150.1416505148957514No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT8640.1337552402950046No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT8440.1306590541770647No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC6930.10728284898661829No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT6790.10511551870406033No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT6570.10170971397432643No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA6510.10078085813894444No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA7650.051.1835981
TCCCCCC4700.047.8184138
TTCCGAT8650.046.0975532
GTATTAA1505.347829E-1043.506061
CCGATCT9600.041.5390324
CTCCCCC5850.038.418217
CGATCTG5500.038.229645
CCCCCCG5700.038.1573039
GTCGCGC4000.036.2494358
ACTCCCC6350.035.3958936
CCATACT6850.033.8680882
GGTCGCG4350.033.3328177
CATACTC7650.032.2242163
TCGCGCG3900.031.6020749
ATACTCC7400.031.3532944
AGGTCGC4200.031.0733536
ACCATAC7000.029.0040421
TACTCCC8250.029.0017975
TCCGATC15000.026.584983
TAAGGTG7100.025.5297495