FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4f11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4f11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1428185
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA23740.16622496385272215No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC23670.16573483127185903No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA19470.13632687642007163No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT18160.1271543952639189No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC18010.12610411116206935No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG17170.12022252019171185No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT16120.112870531478765No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT15350.040.6338274
TTCCGAT16300.038.2642562
CTTCCGA17150.036.7919461
CTAGGGG1851.6391641E-731.3628773
TCCCCCC10000.031.174458
CCCCCCG11000.028.999499
TAGGGGG1606.8130794E-527.1974964
CTCCCCC12050.026.4725637
GTCTACG9900.024.9081441
TATAGGT8750.024.8662823
CCATACT13800.024.7002432
ACTCCCC13050.023.8985086
ATACTCC14600.023.3476224
GTCTAGG2852.7706847E-722.9031541
TACTCCC15500.022.4598675
CCTATAA18900.022.2568571
CGATCTG10450.022.209125
TATAAGC21350.022.0807153
CTATAAG20750.022.019372
CCTGTAC4006.87578E-1021.7579983
Produced by FastQC (version 0.11.2)