FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4e9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4e9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962406
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC12360.12842812700668949No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA11930.12396015818687746No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA10600.1101406267209473No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG10180.10577656415275882No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC9840.10224375159755862No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT14000.038.850414
CTTCCGA14150.037.9280241
TTCCGAT15050.035.6561972
TCGCGCG3800.032.4333849
CCCCCCG5950.031.6798679
CGATCTG8100.029.5454965
TCCCCCC6450.029.2240628
CTCCCCC7200.026.1798887
GTCGCGC3900.026.0249798
GCCCACC6250.025.523329145
ATACTCC8650.025.151714
CCATACT7800.025.1020372
GTATAGA4051.8189894E-1225.0702081
ACCTATA6100.024.967461
GTGTAGG6550.024.3593861
CCTGTAC2401.5939986E-624.173593
TATACAG4201.8189894E-1224.1735885
TACTCCC9500.023.664675
ACTCCCC8350.023.4498276
GGTCGCG4353.6379788E-1223.3327397
Produced by FastQC (version 0.11.2)