FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387720
Sequences flagged as poor quality0
Sequence length151
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG12910.33297224801403075No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC8450.21794078200763436No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG6700.17280511709481067No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA6620.1707417724130816No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC6620.1707417724130816No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG5610.14469204580625195No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC5590.14417620963581967No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC4400.11348395749509955No Hit
GATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGAAG4160.1072939234499123No Hit
ACCTCCGGCTGAGCCGTTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGA3940.10161972557515733No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC3900.10058805323429278No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGTA400.006047335554.376744
TTCCGAT14950.048.4965362
TACTAGT450.00963349948.334882
CTTCCGA15900.046.0549351
TAGCTAT657.329848E-444.616815
CCGATCT17300.041.4897654
TCCGATC18300.039.6187553
CGATCTG8100.039.3839765
CTACTCT750.001482031338.6679044
TAGGAGT1209.546657E-636.251164
ATCTAGC1209.555104E-636.2464877
ACTGGTA1251.2639335E-534.7966278
TATACTC850.00273949434.118743
TAAGGTA1352.138372E-532.2232555
CTTAGAC900.003624943632.2232553
ACTGAAT900.003627234132.21918
TCTTACA1153.1579615E-431.5227492
CTTACAC1153.1579615E-431.5227493
GATCTAG1652.3808607E-630.758566
AGTATAC950.00472302230.5272961
Produced by FastQC (version 0.11.2)