FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4e6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4e6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences689356
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA9350.13563383795890657No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC8300.12040223048758551No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA7570.10981263672180994No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT10600.038.30292
CCGATCT11450.034.8262524
ACAGTGC1902.0771586E-730.5226968
CTATACT1703.0093051E-629.8537314
CTTCCGA14250.029.5095561
CATACTA4800.028.6986772
ACTACCG4750.027.4744115
ATACTAC5550.026.126823
TATAATA2808.183633E-925.8935452
CGATCTG7050.025.7099025
TTAGGAC2901.1888915E-825.0006623
GTGTAGG3501.2914825E-1024.8578011
TACCGTG5900.024.5733577
TGCACTG2751.966273E-723.7279015
CTAAGGC2151.8642642E-523.6052763
TACTCCC6800.023.4565035
GCCTACA3452.9594958E-923.1165542
TGCCTAC3152.8610884E-823.0164851
GTCCTAA2552.7044352E-622.74571
TTAACGT2603.201032E-622.3082854
Produced by FastQC (version 0.11.2)