FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318597
Sequences flagged as poor quality0
Sequence length151
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG3470.10891502430970788No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTT651.092546E-555.7640387
TTCCGAT10650.050.37092
CTTCCGA11600.047.495581
CCGATCT11200.047.2574844
TCCGATC11400.046.4284023
TATAGTG700.001056039641.4247132
TAGACGT800.00203389736.2523164
ACTACAC1352.1367146E-532.224283
GTACTCT900.003623414332.224284
ATAGGGA2101.43245416E-831.0734144
CGATCTA2401.5716068E-930.2102625
TAGGGAA1952.5964255E-729.745495
GTGTAGG1753.7667287E-628.9973011
TATAAGG1000.006071088428.99732
GTAGTAA1000.006071088428.99731
TGCTCTT6400.028.3176772
CGATCTG8000.028.0955455
CCCCTAG1555.4923134E-528.0619031
TTCTAAG2853.2196112E-1027.9798492
ATACAGT1306.470758E-427.8863966
Produced by FastQC (version 0.11.2)