FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4e2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4e2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413248
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG8600.20810748025398793No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC5230.12655838624748336No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGG400.006039199454.3958854
TTCCGAT20650.045.6591762
CTTCCGA20500.045.285681
TCCGATC21950.043.2854083
CCGATCT21950.041.963724
ATCTTAC700.001053927241.444481
GTAGGAT1001.3868997E-436.263923
TAGACGT2103.7471182E-1034.537074
TATAGAG1102.428207E-432.96725
TCTTACA900.00361897332.23462
CGATCTT10050.031.0318155
ACTATGG950.004715251730.538041
TCTGTGC1453.484993E-530.0006288
GATCTTT7500.029.9781746
CGATCTG11500.029.6418155
ATTATGC1000.00605900829.0111353
TCTAGAC1306.461933E-427.8953252
TAAGCGG1306.461933E-427.8953255
TCTTCCG15900.027.8251485
TTAGGAC2405.2363248E-827.1979433
Produced by FastQC (version 0.11.2)