FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4e1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4e1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359832
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4940.13728628915716223No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4620.12839325018341893No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG3700.10282576313390694No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3690.10254785566597745No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTATG451.193191E-464.440442
TACAGTA400.006052568654.3640567
ACATTAC450.00964182248.323618
GAGTTTA1505.347829E-1043.4972881
ACACATA2500.037.713375145
CTTCCGA11600.036.247741
TTCCGAT11650.036.092172
TAAGCAT1854.1691237E-935.2680746
AAGCATA1854.1745807E-935.2631727
TGTAGAC1458.682073E-734.9978182
GTTTAAG2103.765308E-1034.5216563
ACATAGG1102.433956E-432.952491
ACTGTGC1102.4359432E-432.947918
CCTATGG900.003626273232.220223
TCCGATC13150.031.9751933
GATATAC1153.1592598E-431.5197773
CCGATCT13350.031.4961644
CAGACGT1402.742997E-531.0694944
TTAAGCA2101.4353645E-831.069495
ATACTCT950.00472475530.5244144
Produced by FastQC (version 0.11.2)