FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4e12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4e12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511824
Sequences flagged as poor quality0
Sequence length151
%GC59

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA450.0096303948.3410532
CCATTAG803.7242608E-545.3285941
TACAGTA657.3345046E-444.613797
GTATAGA1053.8111339E-641.4432871
TAGAGTG1901.2914825E-1038.163995
CTAGACA800.002034092636.255794
TAACACT800.002034092636.255794
TATACAG800.002034092636.255795
TATGGAC1406.5929635E-736.2557873
TTAGAGT2103.765308E-1034.5293244
TTCCGAT10300.034.49582
CTTCCGA10350.033.635131
TAGTGAG1102.4322147E-432.959812
AATTAGA2755.456968E-1231.6414172
TAATATA1453.4833007E-530.004794
GCCTTAT1000.00606119729.0103021
ATATAAC1000.00606119729.0103021
CATTAGT1050.007698989527.6234592
ACAGTAT1050.007706335727.6180618
GTGTAGG1606.805254E-527.1971571
Produced by FastQC (version 0.11.2)