FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4e11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4e11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1575911
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC24830.15755965914318767No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA23250.14753371224644032No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA19330.12265921108488993No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC18300.11612330899397237No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT17050.10819138898072289No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG16190.10273422801160725No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT16060.1019093083302293No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA20400.041.589961
TTCCGAT21750.039.3419152
CCGATCT21800.038.253764
GTCGCGC8650.032.6835828
TCGCGCG8500.030.7018649
GGTCGCG9550.029.6034557
TATAGGT9850.027.9756473
AGGTCGC10450.027.063326
GTACAGG3451.07320375E-1025.2228951
CCTATAA17450.024.102681
TCCCCCC9700.023.9143968
CGATCTG13350.023.3571575
GGGCGAC5900.023.3444027
TCCGATC37100.023.0643333
GTATTAT6400.022.6611941
CCCCCCG10300.022.5213249
TGCGACC7100.022.4696925
ATAATAC8450.022.3125613
TTGCGAC7250.022.00484
GTATAAG6400.021.5281331
Produced by FastQC (version 0.11.2)