FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4d9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4d9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483134
Sequences flagged as poor quality0
Sequence length151
%GC58

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG8540.17676255448798886No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC5140.10638870375506589No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG5060.104732848443703No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATAC553.2133664E-452.7404483
CTTCCGA13400.044.3721661
TTCCGAT13800.042.5605772
TTATACC700.001055875641.430347145
GTATAAG1252.7119313E-740.6059461
CCGATCT16950.034.2268374
ATAATAC1102.4307257E-432.962783
ATAAGGT2455.4569682E-1132.5591554
TTAATAC900.00362195432.2302743
TACAGGA1352.137783E-532.226942
GGTATTA900.00362379132.2269361
TCCGATC18300.032.0981863
ACCTACA1204.0514953E-430.2127551
TAAGATG1255.1424163E-429.0072485
GAGTTTA2252.8067006E-829.0042441
TGTTATA1000.00606705129.0042422
CATAAGG3102.7284841E-1128.0715313
TATGGAC1050.007695181827.6259483
CTCATAA2903.929017E-1027.5040261
ATAATTC1606.809353E-527.1942963
Produced by FastQC (version 0.11.2)