FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4d8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4d8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1220803
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC17250.14130043913719084No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA16800.1376143407249163No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA14510.11885619547134141No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC13720.11238504492534832No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT12440.10190014277487851No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT12420.1017363161787774No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG12370.10132674968852469No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT12400.042.6873364
TTCCGAT13500.039.744592
CTTCCGA15800.034.4207151
CGATCTG9000.028.1983325
GTATATA6900.027.3237271
CGATCTA3950.025.6997455
GTATAGA5150.023.9363171
TCGCGCG5250.023.475589
CCTACAT4256.184564E-1122.1795273
AGGTGCC10200.022.0337417
TCCCCCC7900.022.0247468
ATTATAC4358.367351E-1121.6696533
GTCGCGC5750.021.4342278
TAAGGTG11600.021.2529325
GTCTAGT4151.0895747E-920.9676041
CCTACAC6650.020.717143
TCCGATC26350.020.6385173
CCCCCCG8450.020.5911799
TAGGACA5403.6379788E-1220.1416654
GGTCGCG6150.020.0401297
Produced by FastQC (version 0.11.2)