FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4d7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4d7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345798
Sequences flagged as poor quality0
Sequence length151
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATAAAGGAGGTAGGGGTGCAGAGACAGCCAGGAGGTTTGCCTAGAAG12460.36032597065338723No Hit
GTACAGGAATATTCACCTGTTGTCCATCGACTACGCCTTTCGGCCTGATC10770.3114535075390835No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG10420.30133199150949397No Hit
CCCTTGAAAGAGTGCGTAATAGCTCACTGATCGAGCGCTCTTGCGCCGAA7640.22093823561732573No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC6400.18507915025535138No Hit
ATCTTAGGCCCTGACTCACCCTCCGTGGACGAACCTTGCGGAGGAACCCT6200.17929542680987168No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC5470.15818483623387064No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA5320.15384704364976085No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC5200.15037680958247301No Hit
GTAGGGGAGCGTTCCGCCTTAGGGGGAAGCAACCGCGCGAGCGGCGGTGG5070.1466173893429112No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT5030.14546064465381522No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG4440.12839866048965004No Hit
GCCTAGAAGCAGCCACCCTTGAAAGAGTGCGTAATAGCTCACTGATCGAG4410.12753110197282808No Hit
GACTCACCCTCCGTGGACGAACCTTGCGGAGGAACCCTTGGGTTTTCGGG4300.12435005407781421No Hit
GATCTTAGGCCCTGACTCACCCTCCGTGGACGAACCTTGCGGAGGAACCC4130.11943388914915645No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC4000.11567446890959462No Hit
GATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGAAG3820.11046911780866286No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA3780.10931237311956692No Hit
GCGTAATAGCTCACTGATCGAGCGCTCTTGCGCCGAAGATGAACGGGGCT3640.1052637667077311No Hit
CTTGAAAGAGTGCGTAATAGCTCACTGATCGAGCGCTCTTGCGCCGAAGA3580.10352864967408718No Hit
ATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAAC3530.10208271881271724No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT10350.067.946862
CTTCCGA10200.067.5245061
CCGATCT12500.056.8399964
GTACATA400.006047550654.3751
CGATCTA2850.053.421055
TCCGATC14050.050.053383
TACTCTG450.00963384748.3333327
GACTAGA450.00963384748.3333321
TACAGGA4500.046.722222
GATCTAA1351.0226358E-842.9629636
ACAGGAA4700.041.6489373
GTCTACA700.001055681341.428572
GTACAGG5050.040.1980171
GGAATAT4750.039.684216
GATCTAG1303.6890196E-739.038466
AGGAATA5050.037.3267335
TGCTCTT5400.034.907412
CAGGAAT5650.034.646024
GACTTAG850.00273951634.117651
ATATAGG850.00273951634.117651
Produced by FastQC (version 0.11.2)