FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1473089
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC19330.1312208563094287No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA19080.1295237422857682No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA16330.11085548802550287No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC16290.1105839497817172No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA20300.045.0121081
CCGATCT23000.040.6753654
TTCCGAT24350.037.5254942
CCCCCCG9200.033.0977449
TCCCCCC9450.032.2221378
TCGCGCG6800.031.9852129
CGATCTG13600.031.4617375
CTCCCCC11050.028.8687047
GTCGCGC7750.028.0644448
GGTCGCG7850.027.7069367
ACTCCCC12500.026.6880846
TATAGGT8550.026.2945163
ATACTCC13850.025.6575934
TCCGATC37150.024.401743
GGGCGAC6000.024.1666037
AGGTCGC9500.023.6650646
TACTCCC14150.023.5760465
CATACTC15450.023.4698933
GTATTGG9750.023.0574841
CCATACT13950.022.8735372
Produced by FastQC (version 0.11.2)