FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4d5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4d5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337695
Sequences flagged as poor quality0
Sequence length151
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATACTCCCCCCAGAACCCAAAAACTTTGATTTCTCGTAAGGTGCCGA4470.13236796517567628No Hit
GAATAATAAAATAGGACGTGCGGTTCTATTTTGTTGGTTTTTGGAATCGC3930.11637720428197042No Hit
GATTAATAGGGACAGTCGGGGGCATTAGTACTCACATGCTAGAGGTGAAA3900.11548882867676454No Hit
CTCGTAAGGTGCCGATCGAGTCAAAAAGATAACATCGACCGATCCCTAGT3590.10630894742297042No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA9500.061.8512271
TTCCGAT9700.059.8280942
CCGATCT10550.054.9996724
TCCGATC11700.049.5937233
TAGACGT901.3170829E-648.353884
ATAGTGC750.001483213738.660198
CGATCTA1956.9339876E-933.475765
TCTTCCG6750.033.3104485
GTGCTCT6650.032.7255171
TATAAGA900.003614690632.24072
GTTATGT900.00361731532.235923
CGATCTC5900.031.9627345
CGATCTT4950.030.7706495
TGCTCTT7100.030.6513672
GTATGTA1204.0388198E-430.2256531
CGATCTG5850.029.7562355
ATACTCC3750.029.0123274
AGAGATC1855.80875E-627.427848
ACCATAC3750.027.0821881
GATCTAA1358.0445805E-426.8672471
Produced by FastQC (version 0.11.2)